The LAMMB provides services to the academia and the industry, with the appropriate quality for the transit of products under development towards the market. There are specialized areas for the protein structural characterization:

  • Analytical area
  • Molecular Biology area
  • 2 Cell culture rooms
  • Purification room
  • Refrigeration cool area and stability chambers
  • Also, an analytical laboratory in the vivarium of the Institute of Biotechnology of the UNAM, for studies in animals

Quality of Biotechnological Drugs

Viruses Screening, Identification and Quantitation.
Viruses screening, identification and quantitation. We have specialized culture chambers and Real-Time PCR equipment. These analyses are made along in collaboration with the INMEGEN.
Quality Tests.
Endotoxin typing, hot cell residual DNA and protein, pH, osmolality, sub-visible particles, appearance, and other quality testing.
Drug Stability.
We have the 2 Binder-rated black test corners of 240 L for assessing stability of the biotechnological drugs.

Characterization and Biological Activity

BIAcore Surface Plasmon Resonance.
We have the BIAcore X equipment for the characterization of biomolecular bonding, ranging from small molecules to crude extracts, viruses, bacteria, and eukaryotic cells, etc.
Host Cell Characterization.
Massive sequencing, quantitation of transcription, and characterization of the host cell. We have the Massive Sequencing and Bioinformatics Units and laboratories in the INMEGEN.
Flow Cytometry.
We have an Accuri C6 Plus flow cytometer for the determination of cells according to their morphological characteristics, the presence of biomarkers, etc.
In Vitro Biological Activity and ELISA Tests.
We have two cell culture sections to perform in vitro biological activity trials and ELISA tests.

Physicochemical Characterization

Protein Mass Spectrometry.
Intact mass, sequencing, disulfide bonds mapping, post-translational modifications (by the Proteomics University Laboratory).
Characterization of the Glycosylation Profile and Identification and Quantitation of Sialic Acids.
Characterization of the glycosylation profile and identification and quantitation of the sialic acids.
Free Sulfhydryls.
We have a validation process to determine free sulfhydryls by using the SpectraMax M3 microplate reader from Molecular Devices.
Fourier-transform Infrared (FT-IR) Transmission Spectroscopy.
We have a Cary 630 FTIR spectrometer unit with a diamond ATR module and a Dialpath module for determining the infrared spectra.
Differential Scanning Calorimetry.
We have a Microcal VP-capillary DSC calorimeter to perform the thermal stability determinations of proteins and their affinities.
Spectrofluorometry and Absorption Spectroscopy.
We have the Scandrop2 and the Pelkin Elmer LS55 for UV / Vis spectrum measurement, in addition to the SpectraMax M3 Microplate Reader that allows measurement of absorbance and fluorescence scans.
Physicochemical Characterization by Chromatographic Methods.
We have chromatographic methods: reducing and non-reducing peptide mapping (RP-HPLC and RP-EC), molecular exclusion chromatography, ion exchange chromatography, among others.
N-Terminal Sequencing (Edman Degradation).
We have an automated protein sequencer PPSQ-31A by Shimadzu Corporation.
Capillary Electrophoresis.
We have the equipment Beckman Coulter PA 800 Plus and the P/ACE MDQ for the determination of the isoelectric point of protein molecules by isoelectric focusing.
SDS-PAGE, Western Blot and Vertical Isoelectric Focusing Gel.
We have systems to perform SDS-PAGE under reducing and non-reducing conditions, and the Ettan IPGphor 3 IEF system for determining the isoelectric point in 1D and 2D gels.
Distant and Near Circular Dichroism.
The Institute of Biotechnology has a Jasco J710 circular dichroism equipment (by Jasco Inc.).
Dynamic Light Scattering and Zeta Potential Determination.
We have the nano Zetasizer equipment by Malvern for determining the particle size and the Zeta Potential.

Research and Development of New Processes

Recombinant proteins production.
We have two cell culture areas and instrumented bioreactors from 0.5L to 50L in disposable reactors.
(c) 2024, Laboratory for the Analysis of Biotechnological Molecules and Drugs (LAMMB)